Polarity Difference and the Presence of Phytoestrogen Compounds Affecting Estrogenic Activity of Peperomia pellucida Extracts

Peperomia pellucida (L.) Kunth has been studied as an anti-osteoporotic agent. However, there is no report about its estrogenic activity, which important for activity. Thus, the aim of this research was to study potency P. extracts. The activity extracts (n-hexane, ethyl acetate, ethanol, and water extracts) using E-screen assay confirmed with a molecular docking simulations. Further, presence phytoestrogen compounds identified thin layer chromatography (TLC), TLC densitometry, high performance liquid chromatography. n-hexane, ethanol at concentration 0.1 μg mL-1 exhibited partial agonist effect, whereas extract showed full agonisteffect similar concentration. This produced through classical ligand-dependent mechanism estradiol. N-hexane acetate antiestrogenic chromatogram evidently depicted quercetin stigmasterol in n-hexane Apigenin apigetrin concentrations 0.239±0.076 1.063±0.156 mg-1 extract, respectively, were present extract. A on estrogen receptors that prefer produce other can both Hence, we suggest bioactive are flavonoids, such apigenin apigetrin. In summary, recommended be used